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Description
Black alder bark (Alnus glutinosa) refers to the lignocellulosic biomass which together with the main cell wall components contains a wide range of polyphenolic extractives including diarylheptanoids which are recognized as a natural antioxidants [1]. Therefore, black alder bark extract is a resource with wide range of possible practical application including antioxidative additives in cosmetics, or/and as a technical antioxidant in polymeric chemistry. In this way microwave assisted water extraction of black alder bark revealed itself as a fast and energy effective methods of extractives isolation. To increase the antioxidative properties of isolated products via increasing of polyphenolic concentration in them the Solid Phase Extraction (SPE) was utilised with two different sorbents of different polarity (Amberlite XAD2 and Amberlite XAD7).
Figure 1. Process workflow for extract fractionation using solid phase extraction
This approach allowed successfully fractionate black alder bark extract with total mass recovery being 95%. Obtained water fractions were enriched with carbohydrates, while obtained ethanol (EtOH) fractions were enriched with phenolic type compounds and showed higher antioxidative activity comparable to that of non-fractionated extracts.
Acknowledgement:This research is funded by the project No. 1.1.1.1/18/A/182
References:
1. Lauberts, M.; Pals, M. Antioxidant Activity of Different Extracts from Black Alder (Alnus glutinosa) Bark with Greener Extraction Alternative. Plants 2021, 10, 2531, doi:10.3390/plants10112531.
