Speaker
Description
Understanding the interplay between compounds in oxidative stress modulation is vital for deciphering mechanisms of neurotoxicity and oxidative stress-related damage. The aim of the current study was to investigate the concentration-dependent effects and interactions of acrylamide (AA), penitrem A (PEN A), and 3-acetyldeoxynivalenol (3-ADON) on lipid peroxidation (LPO) in SH-SY5Y cells.The LPO on SH-SY5Y cells was determined by the TBARS method in the presence of different concentrations of AA, PEN A, and 3-ADON , both individually and in combinations. Results obtained show that after 24h of exposure, the malondialdehyde (MDA) production was significantly increased by each treatment. Results: Concentration-dependent increases in lipid peroxidation were observed with AA, PEN A, and 3-ADON individually and in combination. The triple combination did not result in the highest MDA levels, indicating possible regulatory mechanisms of action. Conclusion: This study underscores the importance of considering compound interactions and concentration-dependent effects in oxidative stress research. The observed interaction patterns highlight the complexity of cellular responses to compound combinations, emphasising the need for comprehensive evaluation in oxidative stress modulation studies. Acknowledgements: Spanish Ministry of Science and Innovation PID2020-115871RB-100, and Conselleria d’Educació, Universitats I Ocupació from Generalitat Valenciana projects AICO/2021/037 and CIAICO2022/199. LB the pre-PhD scholarship (CIACIF/2021/203).
| Presenting author | PhD student Luna Bridgeman |
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