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Gliotoxin (GTX) and Ochratoxin A (OTA), produced by Aspergillus and Penicillium fungi in food and feed, are harmful mycotoxins affecting human and animal health as it has been previously showed in our studies their cytotoxicity in SH-SY5Y neuronal model and its involvement in neurodegenerative diseases. his study investigates their medium inhibitory concentration (IC50) and using Caco-2 cells as a model for the human intestinal epithelium.The IC50 of GTX, OTA, and their combinations was evaluated via the MTT assay at 24h and 48h. GTX demonstrated higher cytotoxicity than OTA across both cell lines and time points, as reflected in lower IC50 values. In SH-SY5Y cells, GTX achieved an IC50 of 1.25 μM after 48 hours, while OTA exhibited a much weaker cytotoxic effect, with an IC50 of 8.25 μM at 24 hours. Similarly, in Caco-2 cells, GTX was more potent, reaching an IC50 of 1.2 μM at 48 hours, compared to OTA, which had IC50 values of 7.9 μM at 24 hours and 5.1 μM at 48 hours.
| Presenting author | Raquel Penalva Olcina |
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